HelixCHO Medium is an advanced serum-free cell culture medium developed for the cultivation of Chinese Hamster Ovary (CHO) cells. This medium is engineered with a unique blend of nutrients to support robust cell growth, maintain high cell viability, and enhance recombinant protein production.
Recommended cell subtypes: CHOZN CHO-K1 (Merck).
Recommended companion media: HelixCHO™ Feed and CDFS36™ Feed Supplement.
This product is intended for research use or further manufacturing, including large-scale biopharmaceutical production. It is not intended for direct human administration or use as a drug substance or drug product.
Key characteristics:
1) Serum-free and chemically defined formulation;
2) Optimized for both growth and production phases of cell culture;
3) Supports high cell densities (>10^7 cells/mL) in fed-batch processes;
4) Improves protein quality attributes including glycosylation profiles;
5) Compatible with various bioreactor configurations and scale-up processes;
6) Delivers high yields across different CHO cell lines while maintaining high cell densities and viability for robust bioprocessing;
7) GMP-ready for seamless R&D-to-GMP transition;
8) Robust intra- and inter-lot consistency (RSD <5%);
9) Flexible packaging formats (dry powder media or liquid).
HelixCHO Medium, when fed HelixCHO Feed and CDFS36 Feed Supplement, outperformed a competitor in CHOZN CHO-K1 mAb expression, showing >100% titer increase on day 14, with higher late-stage VCD and viability.
HelixCHO Medium is ideal for biopharmaceutical companies involved in the development and manufacturing of therapeutic proteins, monoclonal antibodies, and other biological products.
