HelixCHO Feed Medium is an advanced cell culture feed engineered specifically for use with HelixCHO Base Medium in fed-batch cultivation of Chinese Hamster Ovary (CHO) cells. This specialized feed medium is designed to support high-density cell growth and enhance recombinant protein production in complex bioprocesses.
HelixCHO Feed is a chemically defined feed medium formulated for high-density fed-batch processes across multiple Chinese hamster ovary (CHO) cell subtypes. It is hydrolysate-free, animal-origin free (AOF), and does not contain L-glutamine. It contains 84 g/L D-glucose.
Recommended cell subtypes: CHOZN CHO-K1 (Merck), CHO DG44.
Recommended companion media: StarCHO™, VegaCHO™, HelixCHO™ and CDFS36™ Feed Supplement.
This product is intended for research use or further manufacturing, including large-scale biopharmaceutical production. It is not intended for direct human administration or use as a drug substance or drug product.
Key characteristics:
1) Advanced nutrient blend optimized for HelixCHO cell culture system;
2) High concentration of essential nutrients to support extended culture;
3) Enhanced buffering components to maintain optimal pH;
4) Supports complex protein production with desired post-translational modifications;
5) Compatible with scale-up from laboratory to commercial manufacturing;
6) Delivers high yields across different CHO cell lines while maintaining high cell densities and viability for robust bioprocessing;
7) GMP-ready for seamless R&D-to-GMP transition;
8) Robust intra- and inter-lot consistency (RSD <5%);
9) Flexible packaging formats (dry powder media or liquid).
HelixCHO Feed, used in combination with HelixCHO Medium and CDFS36 Feed Supplement, outperformed a competitor in CHOZN CHO-K1 mAb expression, showing >100% titer increase on Day 14, with higher late-stage VCDs and viability.
HelixCHO Feed Medium is recommended for biopharmaceutical companies developing and manufacturing complex therapeutic proteins and monoclonal antibodies.
