DenebCHO Feed Medium is a high-concentration cell culture feed specifically formulated for fed-batch cultivation of Chinese Hamster Ovary (CHO) cells. This advanced feed medium is designed to replenish essential nutrients consumed during cell growth, extending culture duration and significantly enhancing recombinant protein yields.
DenebCHO Feed is a chemically defined feed medium formulated for high-density fed-batch processes
across multiple Chinese hamster ovary (CHO) cell subtypes. It is hydrolysate-free, animal-origin free (AOF), and does not contain L-glutamine. It contains 58 g/L D-glucose.
Recommended cell subtypes: CHO-K1, CHOK1SV GS-KO.
Recommended companion media: AltairCHO™, SagiCHO™, and CDFS36™ Feed Supplement.
This product is intended for research use or further manufacturing, including large-scale biopharmaceutical production. It is not intended for direct human administration or use as a drug substance or drug product.
Key benefits:
1) High nutrient concentration supporting extended fed-batch processes;
2) Balanced formulation preventing nutrient limitation and by-product accumulation;
3) Compatible with various base media and CHO cell lines;
4) Enhances cell viability and productivity during late-stage culture;
5) Supports consistent protein quality attributes;
6) Delivers high yields across different CHO cell lines while maintaining high cell densities and viability for robust bioprocessing;
7) GMP-ready for seamless R&D-to-GMP transition;
8) Robust intra- and inter-lot consistency (RSD <5%);
9) Flexible packaging formats (dry powder media or liquid).
DenebCHO Feed, used in combination with AltairCHO Medium and CDFS36 Feed Supplement, outperformed a competitor in CHOK1SV GS-KO mAb expression, showing 98% titer increase on Day 14, with higher late-stage VCDs and viability.
DenebCHO Feed Medium is ideal for biopharmaceutical manufacturers looking to optimize their fed-batch processes and maximize protein production yields.
